These data have been confirmed by Imaging flow cytometry (Fig. 6B), exactly where there is an apparent shift in the imply fluorescence depth (MFI) of CaSR in oAFMSCs addressed with R2568 (purple curve) when compared to basal affliction (eco-friendly loaded curve)
Given that in these experimental ailments we also performed Oil-crimson O staining, this enables us to affirm that calcimimetics do not induce imaging flow cytometry analysis of CaSR expression in…