Product Name :
TMP778
Description:
TMP778 is a potent and selevtive RORγt inverse agonist, with an IC50 of 7 nM in FRET assay.
CAS:
1422053-04-0
Molecular Weight:
494.58
Formula:
C31H30N2O4
Chemical Name:
2-2-[(S)-(3, 5-dimethyl-1, 2-oxazol-4-yl)(hydroxy)methyl]-1-benzofuran-5-yl-N-[(S)-(2, 4-dimethylphenyl)(phenyl)methyl]acetamide
Smiles :
CC1C=C(C)C=CC=1[C@@H](NC(=O)CC1C=C2C=C(OC2=CC=1)[C@@H](O)C1C(C)=NOC=1C)C1=CC=CC=C1
InChiKey:
DIURRJOJDQOMFC-IOWSJCHKSA-N
InChi :
InChI=1S/C31H30N2O4/c1-18-10-12-25(19(2)14-18)30(23-8-6-5-7-9-23)32-28(34)16-22-11-13-26-24(15-22)17-27(36-26)31(35)29-20(3)33-37-21(29)4/h5-15,17,30-31,35H,16H2,1-4H3,(H,32,34)/t30-,31+/m0/s1
Purity:
≥98% (or refer to the Certificate of Analysis)
Shipping Condition:
Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition :
Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life:
≥12 months if stored properly.
Stock Solution Storage:
0 – 4 oC for 1 month or refer to the Certificate of Analysis.
Additional information:
TMP778 is a potent and selevtive RORγt inverse agonist, with an IC50 of 7 nM in FRET assay.|Product information|CAS Number: 1422053-04-0|Molecular Weight: 494.58|Formula: C31H30N2O4|Chemical Name: 2-2-[(S)-(3, 5-dimethyl-1, 2-oxazol-4-yl)(hydroxy)methyl]-1-benzofuran-5-yl-N-[(S)-(2, 4-dimethylphenyl)(phenyl)methyl]acetamide|Smiles: CC1C=C(C)C=CC=1[C@@H](NC(=O)CC1C=C2C=C(OC2=CC=1)[C@@H](O)C1C(C)=NOC=1C)C1=CC=CC=C1|InChiKey: DIURRJOJDQOMFC-IOWSJCHKSA-N|InChi: InChI=1S/C31H30N2O4/c1-18-10-12-25(19(2)14-18)30(23-8-6-5-7-9-23)32-28(34)16-22-11-13-26-24(15-22)17-27(36-26)31(35)29-20(3)33-37-21(29)4/h5-15,17,30-31,35H,16H2,1-4H3,(H,32,34)/t30-,31+/m0/s1|Technical Data|Appearance: Solid Power|Purity: ≥98% (or refer to the Certificate of Analysis)|Solubility: DMSO : 240 mg/mL (485.26 mM; Need ultrasonic).|Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis|Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.|Shelf Life: ≥12 months if stored properly.|Stock Solution Storage: 0 – 4 oC for 1 month or refer to the Certificate of Analysis.|Drug Formulation: To be determined|HS Tariff Code: 382200|How to use|In Vitro:|It is found that TMP778 at >2.5 μM starts to show toxic effects on cell growth, which however is not RORγt-dependent, since the proliferation of RORγt-deficient T cells cultured under Th17 cell-polarizing conditions is also decreased. Otherwise, these inhibitors do not show inhibitory effects on cell proliferation or RORγt expression or its nuclear translocation, but efficiently inhibited IL-17 production.Obefazimod Data Sheet TMP778 has a much broader dose range and efficiently decreased IL-17 production, consistent with its higher binding affinity for RORγt.NMDA Membrane Transporter/Ion Channel These data indicate that TMP778 is the RORγt inhibitor that most potently reduced IL-17 production.PMID:32813905 |In Vivo:|All three compounds (e.g., TMP778) delay the onset of disease and substantially reduce the severity of disease progression compared to control-treated mice. Consistent with in vitro results, TMP778 treatment causes the most pronounced effect on the disease phenotype. This treatment not only decreases the number of mononuclear cells infiltrating the central nervous system (CNS), but also most strongly reduces the percentage of IL-17+ T cells in the CNS (including IL-17+ IFNγ+). There is no significant change in the percentage IFNγ+ IL-17-T cells in the CNS among all groups, indicating that none of the inhibitors affects Th1 responses. TMP778 strongly inhibits Th17 cell generation, reduces IL-17 production from differentiated Th17 cells, and also dramatically ameliorates the progression of EAE.|Products are for research use only. Not for human use.|