Product Name :
IL-35 (human):Fc (human), (recombinant)
Sequence:
Purity:
≥95% (SDS-PAGE)
Molecular Weight:
~85kDa
Solubility :
Appearance:
Use/Stability :
Stable for at least 6 months after receipt when stored at -20°C.
Description:
Highly pure interleukin involved in T cell regulation. EBI3 (Epstein-Barr virus-induced gene 3 protein; EBV-induced gene 3 protein; IL-27B; IL-27 subunit β) and IL-12A (IL-12 subunit p35; CLMF p35) form the heterodimeric cytokine IL-35 (interleukin-35) involved in T cell regulation. IL-35 is highly expressed in regulatory T cell (Treg) but not in effector or naive T cells.{{763113-22-0} web|{763113-22-0} Technical Information|{763113-22-0} In Vitro|{763113-22-0} supplier} IL-35 is required for the suppressive function of T-reg in vitro and in vivo.
CAS :
Solubility:
Formula:
Additional Information :
| Alternative Name Interleukin-35, EBI3:IL-12A, IL-27B:CLMF p35 | Concentration 0.{{16941-32-5} MedChemExpress|{16941-32-5} Technical Information|{16941-32-5} In stock|{16941-32-5} supplier} 1mg/ml after reconstitution.PMID:30000655 | Endotoxin Content | Formulation Lyophilized. Contains PBS. | MW ~85kDa | Purity ≥95% (SDS-PAGE) | Reconstitution Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein. | Source Produced in HEK 293 cells. Human EBI3 (aa 21-229) linked to human IL-12A (aa 23-219) via the linker GGGSGGGSGGGSGGGS is fused at the C-terminus to the Fc portion of human IgG1 and a FLAG®-tag. | Technical Info / Product Notes Historical data has shown that IL-35 binds to IL-12Rß2, and it has been demonstrated in the literature to inhibit mononuclear cell proliferation.FLAG is a registered trademark of Sigma-Aldrich Co. | UniProt ID Q14213 (IL-27B), P29459 (IL-21A)