S Action.Author ContributionsConceived and created the experiments: HJMMM DFRH JPAK MJPvO CBLMM MS AJN. Performed the experiments: HJMMM RMES DFRH JPAK MS. Analyzed the data: HJMMM RMES JPAK MJPvO CBLMM AJN. Contributed reagents/materials/analysis tools: HJMMM RMES JPAK MJPvO CBLMM MS AJN. Wrote the paper: HJMMM RMES DFRH MJPvO CBLMM MS AJN.
Sun et al. Chemistry Central Journal 2013, 7:114 http://journal.chemistrycentral/content/7/1/RESEARCH ARTICLEOpen AccessD-isoascorbyl palmitate: lipase-catalyzed synthesis, structural characterization and procedure optimization making use of response surface methodologyWen-Jing Sun1,2,3*, Hong-Xia Zhao1, Feng-Jie Cui1,2*, Yun-Hong Li1, Si-Lian Yu2,3, Qiang Zhou2,three, Jing-Ya Qian1 and Ying DongAbstractBackground: Isoascorbic acid is usually a stereoisomer of L-ascorbic acid, and widely applied as a meals antioxidant.DPN However, its extremely hydrophilic behavior prevents its application in cosmetics or fats and oils-based foods. To overcome this dilemma, D-isoascorbyl palmitate was synthesized in the present study for improving the isoascorbic acid’s oil solubility with an immobilized lipase in organic media. The structural information of synthesized solution was clarified making use of LC-ESI-MS, FT-IR, 1H and 13C NMR analysis, and procedure parameters for high yield of D-isoascorbyl palmitate had been optimized by utilizing 1 actor-at-a-time experiments and response surface methodology (RSM). Outcomes: The synthesized solution had the purity of 95 and its structural characteristics were confirmed as isoascorbyl palmitate by LC-ESI-MS, FT-IR, 1H, and 13C NMR analysis. Outcomes from “one actor-at-a-time” experiments indicated that the enzyme load, reaction temperature and D-isoascorbic-to-palmitic acid molar ratio had a important effect on the D-isoascorbyl palmitate conversion rate. 95.32 of conversion rate was obtained by utilizing response surface methodology (RSM) under the the optimized condition: enzyme load of 20 (w/w), reaction temperature of 53 and D- isoascorbic-to-palmitic acid molar ratio of 1:4 when the reaction parameters had been set as: acetone 20 mL, 40 g/L of molecular sieves content material, 200 rpm speed for 24-h reaction time. Conclusion: The findings of this study can grow to be a reference for creating industrial processes for the preparation of isoascorbic acid ester, which could possibly be employed in food additives, cosmetic formulations and for the synthesis of other isoascorbic acid derivatives. Key phrases: Isoascorbyl palmitate, Enzymatic synthesis, Structural characteristic, Response surface methodology, OptimizationBackground D- isoascorbic acid (synonyms: Erythorbic acid) can be a stereoisomer of ascorbic acid (Vitamin C).(-)-(S)-Equol It is a novel meals antioxidant and preservative with fantastic secure overall performance [1].PMID:25105126 D- isoascorbic acid can avoid the food oxidation, inhibit the lower of colour, aroma and flavors, and block the production with the carcinogen ammonium nitrite throughout meals manufacturing procedure. It had been classified as commonly recognized as protected (GRAS) additives by US Meals and* Correspondence: sunwenjing1919@163; fengjiecui@163 1 College of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, P.R. China 2 Jiangxi Provincial Engineering and Technology Center for Food Additives Bio-production, Dexing 334221, P.R. China Complete list of author facts is obtainable in the end in the articleDrug Administration (FDA). Now it might be made use of in processed foods in accordance with Excellent Manufacturing Practice (GMP) [2]. D-isoascorbic.