Ution buffer (1 Triton X-100, 2 mM EDTA, 20 mM Tris-HCl at pH eight.1, 150 mM
Ution buffer (1 Triton X-100, 2 mM EDTA, 20 mM Tris-HCl at pH eight.1, 150 mM NaCl) and immunoprecipitated once more by anti-FLAG (the second ChIP). The recovered DNA was…