Figure 7a,b; 0.8, p 0.001), whereas there was no significant correlation beyellowtail
Figure 7a,b; 0.eight, p 0.001), whereas there was no substantial correlation beyellowtail (Figure 7a,b; 0.eight, p 0.001), whereas there was no important correlation tween cell density and moribundity rates (Figure 7c, d; 0.three, p 0.three). between cell density and moribundity rates (Figure 7c, d; 0.three, p 0.3).Figure 7. Relationship amongst superoxide level (a,b) and cell density (c,d) of Chattonella cultures, andFigure 7.to red sea bream (a,c) and yellowtail (b,d) in the bioassays shown inof Chattonella cultures, toxicity Relationship amongst superoxide level (a,b) and cell density (c,d) Figure 2 (higher DO and toxicity to red sea RLU, relative luminescence(b,d) inData of manage (no Chattonella cell) are DO circumstances) and Figure three. bream (a,c) and yellowtail units. the bioassays shown in Figure 2 (higher alsoconditions) and Figure three. RLU, relative luminescence units. Information of handle (no Chattonella cell) are included. also incorporated.3.4. Fatty Acid Composition three.4. Fatty Acid Composition We analyzed the FA content material of two hugely toxic strains (MRTX-1719 web NIES-1 and Ago03) and two We analyzed the FA content of two hugely toxic strains (NIES-1 and Ago03) and two low-toxicity strains (4KGY and Ago04). The Polmacoxib medchemexpress unsaturated fatty acid (UFA) content ranged low-toxicity strains (4KGY and Ago04). The unsaturated fatty acid (UFA) content material ranged from 26 to 37 mg-1 dry weight (Figure 8a). The UFA content of the least toxic strain, from 26 to 37 g mg-1 dry weight (Figure 8a). The UFA content with the least toxic strain, Ago04, was significantly higher than those of NIES-1 and 4KGY. The saturated fatty acid Ago04, was significantly to 18 mgthose weight (Figure 8b). The SFA of Ago04 was (SFA) content material ranged from 13higher than -1 dryof NIES-1 and 4KGY. The saturated fatty acid (SFA) content material ranged that 13 to 18 g mg-1 FA weight ranged from 39 to 54 mg- was drastically larger thanfrom of NIES-1. Total dry content (Figure 8b). The SFA of Ago04 1 -1 drysignificantly greater than that of Ago04 was substantially larger than thoseto 54 g mg weight. The total FA content of NIES-1. Total FA content material ranged from 39 of NIES-1 dry weight. The total FA content material of Ago04 was drastically larger than these of that NIES-1 and 4KGY. By far the most abundant FA in NIES-1 and 4KGY was 16:0 (palmitic acid), and and 4KGY. Probably the most abundant FA in NIES-1 and 4KGY was (Figure 8c). The contents in Ago03 and Ago04 was 20:five n-3 (eicosapentaenoic acid (EPA)) 16:0 (palmitic acid), and that in Ago03 and Ago04 was 20:5 n-3 (eicosapentaenoic acid (EPA)) (Figure 8c). The contents of 13 FAs differed significantly amongst Chattonella strains. Having said that, correlation analysis of 13 FAs differed drastically correlated together with the moribundity rate correlation analysis showed that no FA was significantlyamong Chattonella strains. On the other hand, of red sea bream showed strains at FA was significantly correlated with all the ( = -0.95 to 0.95, p = red sea exposed to that no 2000 cells mL-1 below low DO conditions moribundity rate of 0.05 -1 bream to 0.89). exposed to strains at 2000 cells mL below low DO situations ( = -0.95 to 0.95, p = 0.05 to 0.89).Antioxidants 2021, 10, 1635 PEER Critique Antioxidants 2021, 10, x FOR12 of 17 12 of50 (a)Unsaturated atty acids ( mg dry weight) Saturated atty acids ( mg dry weight)20 v, w v v w 15 (b) v v v, ww40 30 20 ten 0 NIES-0 4KGY Ago03 Strain Ago04 NIES-1 4KGY Ago03 Strain AgoFatty acids ( mg dry weight)15 (c)NIES-1 4KGY Ago03 Ago Figure 8. Content material of unsaturated fatty acids (a) and saturated fatty acids (.