Tivities of G. braunii leaves.FRAP, TAC and FIC colorimetric assay FRAP procedures are Mouse Epigenetics presented in Table 1. The results DPPH TAC FIC Sample showed both concentration-dependent(mgAAE/g) and purification-enhanced boost within the AOX of (mgAAE/g) the plant. The EtOAc fraction exhibited the top AOX among the partition fractions with EtOH extract 31.21 1.11 g 109.30 0.76 c 281.ten 12.09 e 178.15 3.54 i considerable (p 0.05)75.89 5.05 i of 8.58 and 154.87 inside the DPPH and FIC assays, reIC50 values a d n-Hexane ten 172.88 1.12 450 h c, f k spectively. EtOAc eight.58 1.39 178.64 2.04 485.81 50.41 154.87 6.54 b f (p 0.05) 0.97 ethan283.83 23.01 j The former was significantly L-ascorbic acid (AA) with an IC50 n-BuOH 175.38 much better 298.79 32.51 e 16.06 0.52 athe EtOAc fraction as an j b value of 11.38 . 86.97 energy (FRAP) and 18.23 The 24.74 Aqueous ten 49.94 capacity (TAC) of 450 h a k b 1 ten one hundred 12.02 1.37 450 h antioxidant were one-sixth and halved, respectively, when compared with AA. This img c k two 347.26 1.43 43.91 1.20 100 450 h plies that the fraction was able to perform its antioxidant role by hydrogen atom transfer h h three the stable DPPH absolutely free radical, single electron transfer (SET)din the FRAP and TAC 61.53 1.01 651.77 7.98 77.72 0.39 450 h (HAT) to g 4 3.09 i 6.38) chelation 702.89 the case of FIC assay [25,26].410.64 indings 0.48 b, 115.23 4.12 f assays and by metal (Fe as in These 8.62 5 15.78 0.41 e 121.87 2.73 g 752.76 13.51 k 389.92 four.76 f corroborate the reported in vivo antioxidant activity from the EtOAc fraction in mice [10]. 6 0.86 0.37 a, 199.63 5.67 c 720.47 ten.08 j 161.57 3.79 h As 7 presented in Table 1, compounds 1 hadk low 81.12 hile four have been active. The AOX, 2.22 e k one hundred 764.09 10.12 450 h ketone group at C-3 position in phyllanthone 20.31 h have helped tof confer one-third fold two might 115.76 three.65 i 8 70.64 2.90 634.84 255.53 11.71 d d FRAP activity, while globraunone 3 16 manufacturer proved -to be essentially the most active amongst the terpenesaisoPositive control 13.21 two.56 11.38 0.45 lated partly as a consequence of the imply SEM, significant (p 0.0001) compared with finding is in Values n = 3, values presented as thepresence of ketone and hydroxyl groups. This constructive handle.consowith distinctive alphabets Baccouri and Rajhi [27] on the significance of hydroxyl, carbonyl and nance with that of in superscript are significantly diverse at p 0.05; isolated compounds (1). olefine for the antioxidant activities of compounds as lead molecules for drug discovery. The 6 demonstrated the ideal AOX among the isolated compounds. Its AOX was 12 Guaiacolformer was drastically (p 0.05) superior than L-ascorbic acid (AA) with an IC50 value of 11.38 . The energy (FRAP) and capacityAA within the DPPH assay, although, because the times better as a hydrogen-atom-donor (HAT) than (TAC) from the EtOAc fraction in an antioxidant have been one-sixth and halved, respectively, when compared(SET) AA. This implies FRAP assay, it was 0.76-times as good as a single-electron-donor with when compared that the fraction was able to carry out its antioxidant function by hydrogen atom transfer (HAT) with AA. to theThis could possibly be as a result of its pi electron-rich benzene ring and hydroxyl group, which can steady DPPH totally free radical, single electron transfer (SET) in the FRAP and TAC assays two and by metal HAT) chelation as within the case of FIC assay [25,26]. HAT is actually a one-step reaction exhibit each (Fe and SET mechanisms of antioxidant action. These findings corroborate the phenolic O-H to effect bond dissociation enthalpy fractionSET is actually a.