Ry of Ukraine had began in 600 Th years last century from Crimea. These days Ambrosia is registered in 234 regions of Ukraine like the Central Part, the collecting area are 1,328,377,863 ha. In Western Ukraine it appeared additional than 20 years ago and its region escalating speedily. Aim To evaluate the capabilities of sensitization to Ambrosia in individuals from Lviv (Western Ukraine) and Kyiv regions (Central Ukraine) and toanalyze the efficacy of AIT with Extract of Ambrosia (Diater Laboratories, Spain). Techniques: 586 individuals aged 58 have been examined in Kyiv 327 (55.8 ) and 259 (44.2 ) in Lviv area. SPT was performed by Extract “Ambrosia”. The individuals had been examined to undergo molecular diagnostics making use of ImunoCAP (Phadia) to recognize Tetrahydrofolic acid Epigenetic Reader Domain significant (n Amb a1) allergen. The SLIT was carried out with a mixture of Ambrosia. Benefits: The prevalence of Ambrosia sensitization diagnosed in 25 (9.6 ) persons within the Lviv area. 3 (12.0 ) youngsters moved in the Crimea and 22 (88.0 ) adults was born and have been living in Western Ukraine. Sensitization to Ambrosia main allergen (n Amb a1) was detected in 23 (92.0 ) persons. Withal, sensitization to Ambrosia (SPT) in sufferers within the Kyiv region was determined two.5 times greater: good SPT had been detected in 80 (24.5 ) individuals (28 (35.0 ) young children and 52 (65.0 ) adults). The accurate sensitization has been confirmed in 88.0 of men and women. SLIT had been prescribed inside the sufferers from both regions. The efficacy of SLIT was assessed by a visual analogue scale (VAS-up). An assessment of SLIT 2-hydroxymethyl benzoic acid custom synthesis showed that there was a considerable decrease in the severity of symptoms inside the study groups as after 2 years of remedy (94.1 and 94.5 , respectively). Conclusions: A higher level of sensitization to key allergen (n Amb a1) Ambrosia in kids and adults in Ukraine leads to a significant enhance in allergic pathology. High efficacy from the SLIT offers the possibility of relative control with the prevalence of extreme kind of allergic ailments. Poster Discussion Session I Topic 2: The basis of allergenicity P10 Identification of IgEbinding epitopes around the surface with the nonspecific lipid transfer protein Art V 3 Sabrina Wildner1, Iris Grie er2, Martina Dimuzio2, Eva Vejvar2, Sara Huber2, Michael Hauser2, Adriano Mari3, Mario Schubert2, Brandstetter Hans2, Gabriele Gadermaier2 1 University of Salzburg, Christian Doppler Laboratory for Biosimilar Characterization, Salzburg, Austria; 2University of Salzburg, Division of Molecular Biology, Division of Allergy and Immunology, Salzburg, Austria; three Linked Centers for Molecular Allergology, Rome, Italy Correspondence: Sabrina Wildner [email protected] Clinical Translational Allergy (CTA) 2018, eight(Suppl 1):P10 Background: Pollen of Artemisia vulgaris (mugwort) are a vital elicitor of allergic reactions in late summer season and autumn. Art v 3 is definitely an allergen of mugwort pollen which belongs towards the non-specific lipid transfer protein (LTP) family. The aim with the study should be to solve the structure of Art v three and to recognize the structural epitopes of Art v 3 using murine monoclonal antibodies. Procedures: Recombinant non-labeled and double-labeled (13C15N) Art v three.0201 had been expressed in E. coli and purified making use of cation exchange chromatography. The three-dimensional structure of Art v 3 was solved by X-ray crystallography and resonance assignment was obtained by NMR spectroscopy. In addition, three Art v 3-specific murine monoclonal IgG antibodies (mAbs) have been created in hybridoma cells a.