Epresentative of OsGRF4 promoter haplotypes A, B and C (see main text) are shown. e, OsGRF4 mRNA abundance in numerous rice varieties under the high N situations (1.25 mM NH4NO3), OsGRF4 promoter haplotypes as indicated. Abundance information is all relative to the abundance of rice Actin2 mRNA. Data shown as mean s.e.m. (n = three). Distinct letters Bretylium tosylate denote important differences (P 0.05, SB-612111 In Vitro Duncan’s various range test). f, Comparisons of OsGRF4 mRNA abundance in selected rice varieties grown in among high (HN, 1.25 mM NH4NO3) and low (LN, 0.375 mM NH4NO3) N situations. Information shown as imply s.e.m. (n = three). Abundance information is all relative to that in HN (set to a single). P 0.05 as in comparison with HN by two-sided Student’s ttest. g, Relative abundances of rice OsmiR396 members of the family in NJ6 plants grown at various levels of N provide (0.15N, 0.1875 mM NH4NO3; 0.3N, 0.375 mM NH4NO3; 0.6N, 0.75 mM NH4NO3; 1N, 1.25 mM NH4NO3), shown relative to abundance in plants grown in 1N circumstances (set to one particular). Information shown as imply s.e.m. (n = three). Various letters denote important differences (P 0.05, Duncan’s multiple variety test).Europe PMC Funders Author Manuscripts Europe PMC Funders Author ManuscriptsNature. Author manuscript; readily available in PMC 2019 February 15.Li et al.PageEurope PMC Funders Author Manuscripts Europe PMC Funders Author ManuscriptsExtended Data Figure two. Comparisons NJ6, NJ6-sd1 and NJ6-sd1-OsGRF4ngr2 isogenic line traits reveals that OsGRF4 regulates expression of NH4+ metabolism genes.a, Mature plant height. Information shown as mean s.e.m. (n = 16). Various letters denote important variations (P 0.05, Duncan’s many range test). b, The amount of tillers per plant. c, The number of grains per panicle. Information shown as imply s.e.m. (n = 16). Diverse letters denote important variations (P 0.05, Duncan’s many range test). d, Flag-leaf width. Information shown as imply s.e.m. (n = 16). Unique letters denote significant differences (P 0.05, Duncan’s a number of variety test). e, Culm (stem) width expressed as diameter in the uppermost internode. Information shown as mean s.e.m. (n = 16). Diverse letters denote considerable differences (P 0.05, Duncan’s several range test). f, Grain yield per plant. Information shown as imply s.e.m. (n = 220). Unique letters denote substantial variations (P 0.05, Duncan’s various range test). g, Relative root abundance of OsAMT1.two mRNA in NILs, genotypes as indicated. Abundance shown relative to that in NJ6 plants (=1). Information shown as mean s.e.m. (n = three). Various letters denote significant differences (P 0.05, Duncan’sNature. Author manuscript; accessible in PMC 2019 February 15.Li et al.Pagemultiple variety test). h, Root glutamine synthase (GS) activities. Information shown as imply s.e.m. (n = three). Unique letters denote important differences (P 0.05, Duncan’s multiple range test). i, Relative shoot abundance of OsFd-GOGAT mRNA. Abundance shown relative to that in NJ6 plants (=1). Data shown as imply s.e.m. (n = three). Unique letters denote substantial differences (P 0.05, Duncan’s multiple range test). j, Shoot glutamine synthase (GS) activities. Data shown as mean s.e.m. (n = 3). Diverse letters denote important differences (P 0.05, Duncan’s various variety test). k-n, Flag-OsGRF4 mediated ChIP-PCR enrichment (relative to input) of GCGG-containing promoter fragments (marked with ) from OsAMT1.2, OsGS2, OsNADH-GOGAT2 and OsFd-GOGAT promoters. Diagrams depict putative OsAMT1.two, OsGS2, OsNADH-GOGAT2 and OsFd-GOGAT promoters.