S (TRECs) as biomarkers for de novo T cell synthesis using a quantitative polymerase chain reaction-enzyme linked immunosorbent assay (PCR-ELISA). J Immunol Methods 2000, 237:187-97. 10. Douek D, Vescio RA, Betts MR, Brenchley JM, Hill BJ, Zhang L, Berenson JR, Collins RH, Koup RA: Assessment of thymic output in adults after haematopoietic stem-cell transplantation and prediction of T-cell reconstitution. Lancet 2000, 355:1875-81. 11. Svaldi M, Lanthaler AJ, Dugas M, Lohse P, Pescosta N, Straka C, Mitterer M: T-cell receptor excision circles: a novel prognostic parameter for the HIV-1 integrase inhibitor 2 cost outcome of transplantation in multiple myeloma patients. Br J Haematol 2003, 122:795-801. 12. Poulin JF, Sylvestre M, Champagne P, Dion ML, Kettaf N, Dumont A, Lainesse M, Fontaine P, PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28380356 Roy DC, Perreault C, S aly RP, Cheynier R: Evidence for adequate thymic function but impaired naive T-cell survival following allogeneic hematopoietic stem cell transplantationLi et al. Journal of Translational Medicine 2010, 8:47 http://www.translational-medicine.com/content/8/1/Page 8 of13.14.15.16.17.18.19.20.21.22.23. 24.25.26.27. 28.in the absence of chronic graft-versus-host disease. Blood 2003, 102:4600-7. Przybylski GK, Kreuzer KA, Siegert W, Schmidt CA: No recovery of T-cell receptor excision circles (TRECs) after non-myeloablative allogeneic hematopoietic stem cell transplantation is correlated with the onset of GvHD. J Appl Genet 2007, 48:397-404. Poulin JF, Viswanathan MN, Harris JM, Komanduri KV, Wieder E, Ringuette N, Jenkins M, McCune JM, S aly RP: Direct evidence for thymic function in adult humans. J Exp Med 1999, 190:479-86. Li Y, Chen S, Yang L, Yin Q, Geng S, Wu X, Schmidt CA, Przybylski GK: TRAV and TRBV repertoire, clonality and the proliferative history of umbilical cord blood T cells. Transplant Immunol 2007, 18:151-8. Petridou E, Klimentopoulou AE, Moustaki M, Kostrikis LG, Hatzakis A, Trichopoulos D: Recent thymic emigrants and prognosis in T- and B-cell childhood hematopoietic malignancies. Int J Cancer 2002, 101:74-7. Haining WN, Evans J, Seth N, Callaway G, Wucherpfennig K, Nadler L, Guinan E: Rapid T Cell Response to Vaccination Can Occur without Antibody Response in Children Post HSCT. Blood 2004, 104(11):614a. Li Y, Yin Q, Yang L, Chen S, Geng S, Wu X, Zhong L, Schmidt CA, Przybylski GK: Reduced levels of recent thymic emigrants in acute myeloid leukemia patients. Cancer Immunol Immunother 2009, 58:1047-55. Geng SX, Li YQ, Chen SH, Yang LJ, Yin QS, Wu XL, Zhang XL: Peripheral blood naive T cell level and its T cell receptor Vbeta repertoire usage profile in patients with chronic myelogenous leukemia. Zhonghua Xue Ye Xue Za Zhi 2005, 26:413-6. (Chinese) Kimmig S, Przybylski GK, Schmidt CA, Laurisch K, Mowes B, Radbruch A, Thiel A: Two subsets of naive T helper cells with distinct T cell receptor excision circle content in human adult peripheral blood. J Exp Med 2002, 195:789-94. Wang L, Zhu K, Zha X, Chen S, Yang L, Chen S, Li Y: Evolution of T-cell clonality in a patient with Ph-negative acute lymphocytic leukemia occurring after interferon and imatinib therapy for Ph-positive chronic myeloid leukemia. J Hematol Oncol 2010, 3:14. Rossi E, Matutes E, Morilla R, et al.: Zeta chain and CD28 are poorly expressed on T lymphocytes from chronic lymphocytic leukaemia. Leukemia 1996, 10:494-7. Chen S, Yang L, Chen S, Li Y: TCR chain expression in T cells from patients with CML. Hematology 2009, 14:95-100. Farace F, Orlanducci F, Dietrich PY, Gaudin C, Angevi.