Tion and preliminary analysis [37]. Interviewers continued to interview participants until no new major themes emerged from the interviews and theoretical saturation had been reached [37]. Each focus group lasted approximately 90 minutes. The focus group moderator (KK) used a modified guide to lead discussions with healthcare workers about the clinical practices associated with disclosure, the role they played in the process, and the challenges that arise with the current system and guidelines. With permission, interviews and focus groups were audiorecorded and later transcribed verbatim. One interview participant did not consent to audio recording and the interviewer took hand-written notes instead. Additional hand-written notes were taken during focus groups to guide the data analysis process by giving an indication of non-verbal communication and group dynamics during these discussions [38].Methods SettingThe study was conducted from December 2012 to July 2013 at the HIV clinic of Parirenyatwa Hospital in Harare, Zimbabwe: one of the largest HIV treatment facilities in the country. The center has a specifically tailored adolescent clinic on a weekly basis and an enrolment of 1473 HIV-positive adolescents. Adolescents on treatment who are aware of their status also have access to a clinic-based peer support group.Data AnalysisAgain using a constant-comparative approach [37], two members of the research team (KK, LC) with different cultural and professional backgrounds independently reviewed and coded transcripts. Through a line-by-line analysis of transcripts, the two coders assigned labels to contiguous segments of text pertaining to similar ideas (codes). The coders met several times to reconcile emerging codes, allowing them to come up with the first set of 20 codes. A computerized database in MS Excel was then used to aid the data organization and retrieval process. A research team with CV205-502 hydrochloride biological activity experience in medical anthropology, qualitative health research, social work, and adolescent HIV (KK, ZM, LC, RF, CN, MB) met several times in order to organize and discuss emerging themes and concepts until a consensus was reached on the final coding framework. Researchers developed hypotheses on an inductive basis and performed axial coding [39]Study Design and SampleIn this qualitative study we used a combination of in-depth interviews with perinatally-infected adolescents aged 16?0 (n = 31; mean age = 17) who were aware of their HIV status and two focus group discussions with healthcare workers (n = 15) at the clinic. Adolescents were selected using purposive sampling [35] at their weekly peer support group, which normally has 30?PLOS ONE | www.plosone.orgHIV Status Disclosure to Oroxylin A molecular weight AdolescentsTable 1. Interview Guide.1. How has your day been? 2. How did you get into the clinic today? 3. How are you feeling today? Is there anything with your health that is bothering you? 4. Did you have any idea of your status before the first time that it was explained to you? How did you obtain this knowledge? 5. Did you ever take pills without knowing what they were for? 6. Why were you tested? Did you know you were being tested at the time? 7. Think back to the time you were told about your illness Can you tell me what happened. Who told you that you were HIV-positive? Where were you told? Describe the setting. What was your initial reaction? 8. What did you think of the way you were told? Was it the right way? Would you have preferred anything else? Would you.Tion and preliminary analysis [37]. Interviewers continued to interview participants until no new major themes emerged from the interviews and theoretical saturation had been reached [37]. Each focus group lasted approximately 90 minutes. The focus group moderator (KK) used a modified guide to lead discussions with healthcare workers about the clinical practices associated with disclosure, the role they played in the process, and the challenges that arise with the current system and guidelines. With permission, interviews and focus groups were audiorecorded and later transcribed verbatim. One interview participant did not consent to audio recording and the interviewer took hand-written notes instead. Additional hand-written notes were taken during focus groups to guide the data analysis process by giving an indication of non-verbal communication and group dynamics during these discussions [38].Methods SettingThe study was conducted from December 2012 to July 2013 at the HIV clinic of Parirenyatwa Hospital in Harare, Zimbabwe: one of the largest HIV treatment facilities in the country. The center has a specifically tailored adolescent clinic on a weekly basis and an enrolment of 1473 HIV-positive adolescents. Adolescents on treatment who are aware of their status also have access to a clinic-based peer support group.Data AnalysisAgain using a constant-comparative approach [37], two members of the research team (KK, LC) with different cultural and professional backgrounds independently reviewed and coded transcripts. Through a line-by-line analysis of transcripts, the two coders assigned labels to contiguous segments of text pertaining to similar ideas (codes). The coders met several times to reconcile emerging codes, allowing them to come up with the first set of 20 codes. A computerized database in MS Excel was then used to aid the data organization and retrieval process. A research team with experience in medical anthropology, qualitative health research, social work, and adolescent HIV (KK, ZM, LC, RF, CN, MB) met several times in order to organize and discuss emerging themes and concepts until a consensus was reached on the final coding framework. Researchers developed hypotheses on an inductive basis and performed axial coding [39]Study Design and SampleIn this qualitative study we used a combination of in-depth interviews with perinatally-infected adolescents aged 16?0 (n = 31; mean age = 17) who were aware of their HIV status and two focus group discussions with healthcare workers (n = 15) at the clinic. Adolescents were selected using purposive sampling [35] at their weekly peer support group, which normally has 30?PLOS ONE | www.plosone.orgHIV Status Disclosure to AdolescentsTable 1. Interview Guide.1. How has your day been? 2. How did you get into the clinic today? 3. How are you feeling today? Is there anything with your health that is bothering you? 4. Did you have any idea of your status before the first time that it was explained to you? How did you obtain this knowledge? 5. Did you ever take pills without knowing what they were for? 6. Why were you tested? Did you know you were being tested at the time? 7. Think back to the time you were told about your illness Can you tell me what happened. Who told you that you were HIV-positive? Where were you told? Describe the setting. What was your initial reaction? 8. What did you think of the way you were told? Was it the right way? Would you have preferred anything else? Would you.