Cells ended up then collected at 0uC, resuspended in 1 ml of Quit Buffer (150 mM NaCl, 50 mM NaF, ten mM EDTA, 1 mM NaN3) , briefly centrifuged at…
Cells have been then gathered at 0uC, resuspended in one ml of Stop Buffer (one hundred fifty mM NaCl, fifty mM NaF, ten mM EDTA, 1 mM NaN3) , briefly…
Asterisks denote cross- reacting proteins. The migration of molecular size standard is indicated on the remaining. (TIF)Figure S4 Ubiquitin conjugates are enriched in the ER fraction of cdc48-three. CDC48 and…