Target cell populations were sorted directly onto MEF layers (ARIA plate holder at 37uC) at between 26103 and 56104 cells/well in a 6-well plate containing HUESM plus 20 mM Y-27632
Stained cells ended up washed after with iPSC staining buffer and sorted quickly on a 5 laser BD Biosciences ARIA-IIuTM SOU Mobile Sorter utilizing ``gentle FACS'' sorting circumstances based on…