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Mitochondria/Cytosol fractionation kit
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The core kit contains unique formulations of buffers and reagents that allow isolation of mitochondria and cytosol in only two easy steps. No ultracentrifugations are required. No toxic chemicals are involved.
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| Application Notes For the effective isolation of a highly enriched mitochondrial fraction from the cytosolic fraction of various mammalian cells including both apoptotic an non-apoptotic cells.{{146426-40-6} MedChemExpress|{146426-40-6} Biological Activity|{146426-40-6} In Vitro|{146426-40-6} supplier} The enriched mitochondrial and cytosolic fractions can be used to study apoptosis and signal trasduction pathways by detecting translocations of factors of interest between the two fractions by ELISA, Western blot or other assays.{{149647-78-9} web|{149647-78-9} Technical Information|{149647-78-9} In stock|{149647-78-9} custom synthesis} | Contents 10ml Mitochondria Extraction Buffer, 20ml 5X Cytosol Extraction Buffer, 110µl DTT (1M), 1 vial Protease Inhibitor Cocktail.PMID:30570986 | Quantity ~100 tests. | Species Reactivity Mammalian