Nts have an IgE response to extracts from I. ricinus and 42 from A. americanum. Amongst the US patients 89 had IgE reactivity to extracts from I. ricinus in comparison to 30 from A. americanum. There was moderate to high correlation involving the IgE levels to -Gal and extracts from adult ( = 0.54 for Swedish sera and 0.87 for USA sera) and larvae from I. ricinus ( = 0.54 for Swedish sera and = 0.88 for US sera). There was no correlation involving IgE levels to -Gal and also a. americanum larvae extract. In 1D-immunoblotting a wide-range of protein bands (2550 kDa) had been identified by the Swedish and American serum pools. The A. americanum larvae showed to have less IgE-binding protein bands than I. ricinus. The presence of -Gal in both tick species was further supported by IgE inhibition using thyroglobulin. All patients tested showed allergenic activityagainst extract from adult I. ricinus within the basophil activation assay. Deglycosylation on the I. ricinus extract reduced the allergenic activity. Conclusions: The results assistance the powerful relationship with tick bites for the production of IgE to -Gal. Furthermore, that tick proteins have allergenic activity. Even so, the IgE reactivity to -Gal containing tick extract seem to differ amongst species and stadium in the tick life cycle, suggesting that ticks might obtain the -Gal epitope from their mammalian hosts. P28 Pru P X is actually a new allergen of peach tree pollen involved in sensitization Maria Luisa SomozaAlvarez Hospital Infanta Leonor, Madrid, Spain Correspondence: Maria Luisa SomozaAlvarez [email protected] Clinical Translational Allergy (CTA) 2018, 8(Suppl 1):P28 Background: Peach tree pollen has ben identified as relevant in locations of peach tree cultivar. Immediately after olive and grass, it really is the third 1 inducing sensitisation in these areas. Our aim was to study if peach tree pollen contain other allergens that could induce sensitisation as well as Pru p 3. Approaches: Skin tests with peach pollen extracts have been produced in subjects with seasonal symptoms during the period of production of this pollen in an region of higher exposure. Sera from good skin tests instances were obtained and SDS-PAGE and immunoblotting evaluation was produced. Benefits: Employing pool of sera of Bucindolol site mono-sensitized situations adverse to Pru p three and other pollens numerous bands had been identified that corresponded to 45, 25 and 15 kD. We named the 15D band as Pru p X. This protein and Pru p three in 110 situations skin test positive to peach pollen. The 40 had been prick positive to Pru p X along with the 35 to Pru p 3. The 12 had been constructive to both and in the remaining cases with skin test optimistic to peach pollen each had been damaging. Conclusions: Peach pollen has various allergens that can be involved in the induction of sensitisation and allergy in extremely exposed populations. From these we recognize the Pru p X that has not been previously recognized. Simply because subjects had been also optimistic to Pru p three, the respiratory tract could be a pathway of sensitisation to this pan-allergen. The clinical relevance of those findings is below evaluation. P29 Class III chitinase is a new IgE binding protein identified in pomegranate Lisa Tuppo1, Ivana Giangrieco1, Claudia Alessandri2, Maurizio Tamburrini1, Chiara Rafaiani2, Michela Ciancamerla2, Adriano Mari2, Maria Antonietta Ciardiello1 1 Istituto di Bioscienze e Biorisorse, Consiglio Nazionale delle Ricerche, Naples, Italy; 2Centri Associati di Allergologia Molecolare CAAM, Rome, Italy Correspondence: Adriano Mari adriano.mari@c.