Tubes were being then centrifuged and the supernatant was transferred to a thoroughly clean tube, combined with phenylalanyl chloromethyl ketone (TPCK)-dealt with trypsin (Sigma), solved by SDS Web page, and transferred to nitrocellulose paper

Gentle-dependent activation of coral G proteins by acropsins. In vitro translated Ga subunits were reconstituted with membranes of acropsin-expressing HEK293t cells as described in Components and Approaches. The proteins were…